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When you record in Studio One 4, or any DAW, it is normal to record your audio signal without any processing. This allows you to apply FX after you've recorded the sound. From here you can choose to keep your processed audio, or make adjustments before committing these changes to your original audio source.
Before recording, you can use the INSERT section of your Audio Track with your guitar on it to add AmpliTube 4 inside your DAW.
From here you can both monitor your processed signal during and after recording with FX. Using this process you can also automate the controls in your performance. For example, adding a distortion pedal in just your solo, or turning on and controlling WAH during a bridge.
Here is a GIF example of the quick process and a YouTube video which may help further.
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